Suspect Screening and Semi-Quantification of Macrolide Antibiotics in Municipal Wastewater by High-Performance Liquid Chromatography—Precursor Ion Scan Tandem Mass Spectrometry

Macrolides are widely used in medicine and veterinary medicine, the leading antibiotics terms of consumption. The release macrolides their metabolites into environment through municipal wastewater can have an adverse effect on aquatic ecosystems human health. In present study, a method for non-targeted screening semi-quantitative determination macrolide derivatives based combination chromatographic separation tandem mass spectrometric detection precursor ion scan (PrecIS) mode has been proposed. Product ions with m/z 158 174 related to specific desosamine fragments were as diagnostic PrecIS structures without (14- 15-membered macrocycles) (16-membered macrocycle) glycosylated moiety, respectively. optimized solid phase extraction procedure HPLC-MS/MS analysis allowed suspect limits range 4–150 ng L−1. developed approach made it possible detect tentatively identify 17 compounds belonging class, including azithromycin, clarithromycin, josamycin 14 total concentration 1450